Drug Sensitivity Testing: Once bacterial growth is detected, the bacteria are exposed to five first-line anti-TB drugs (Isoniazid, Rifampicin, Ethambutol, Pyrazinamide, and Streptomycin) to determine their sensitivity.
Understanding AFB 5 Drug Sensitivity (First Line) for Bactec
What It Is: AFB 5 Drug Sensitivity (First Line) for Bactec is a diagnostic test used to determine the sensitivity of Mycobacterium tuberculosis to five first-line anti-TB drugs. This test helps guide the appropriate treatment regimen for tuberculosis (TB) patients.
Why It's Done:
Guiding Treatment: The primary purpose of this test is to identify which first-line anti-TB drugs are effective against the TB bacteria in a patient's sample.
Personalized Therapy: Helps tailor the treatment plan to the specific drug sensitivities of the TB bacteria, improving treatment outcomes.
Sample Types:
Sputum: Most commonly used sample for TB testing.
Blood: To detect TB bacteria in the bloodstream.
Urine: To identify TB bacteria in the urinary tract.
Fluid: To test for TB in other bodily fluids, such as cerebrospinal fluid or pleural fluid.
Pus: To identify TB bacteria in pus from abscesses or wounds.
How the Test Works:
Sample Collection: A sample, such as sputum, blood, urine, fluid, or pus, is collected from the patient.
Inoculation: The sample is inoculated into a Bactec MGIT tube containing a liquid medium that supports the growth of mycobacteria.
Incubation: The tube is placed in a Bactec MGIT instrument, which continuously monitors the sample for bacterial growth.
Drug Sensitivity Testing: Once bacterial growth is detected, the bacteria are exposed to five first-line anti-TB drugs (Isoniazid, Rifampicin, Ethambutol, Pyrazinamide, and Streptomycin) to determine their sensitivity.
Detection: The instrument measures the response of the bacteria to each drug, indicating which drugs are effective in inhibiting bacterial growth.